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- W2348211046 abstract "UCP genes are newly discovered genes that can increase the energy expenditure and involve the metabolism of fat and the regulation of energy.In this paper RT-PCR method was developed,which could be used for the semiquantitative analysis of UCP-2 mRNA in pig fat.18s rRNA was used as interal standard.Total RNAs were extracted from fat tissue of pig.The mRNA was reverse-transcripted and the cDNA fragments of UCP-2 mRNA and 18s rRNA gene were amplified by RT-PCR using specific primers.The integrated optical density(IOD) was showed by scanning the electrophoresis bands of PCR products with VDS system.By studying the linear range of PCR amplification,the optimal cycle numbers and Mg~(2+) concentration were determined.The relative concentration of UCP-2 mRNA can be calculated by the IOD ratio of PCR products derived from UCP-2 mRNA and 18s rRNA." @default.
- W2348211046 created "2016-06-24" @default.
- W2348211046 creator A5047581480 @default.
- W2348211046 date "2006-01-01" @default.
- W2348211046 modified "2023-09-23" @default.
- W2348211046 title "Semiquantitative analysis of UCP-2 mRNA in fat tissue of pig by RT-PCR" @default.
- W2348211046 hasPublicationYear "2006" @default.
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