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- W2348236180 abstract "B cell antigenic epitope for vaccine candidate SirH against Staphylococcus aureus was predicted.The sirH gene was amplified from genomic DNA of Staphylococcus aureus clinical strain by PCR.The gene fragment was inserted into the expression vector pET-28a(+)to build pET28a-sirH.The recombinant plasmid was identified by restriction analysis and sequencing.The nucleotide and deduced amino acid sequences of sirH gene were compared with those of Staphylococcus aureus available from the GenBank database,based on which the secondary structure of flexible region,hydrophilicity,surface probability and antigenicity index for SirH protein were analyzed by bioinformatics software.Combined with these results,the N-terminal No.49-57,62-75,83-123,128-162,194-208,242-252,334-350,401-415,452-543,550-564,573-622 were be the possible B cell antigenic epitopes for SirH.B cell antigenic epitope for SirH against Staphylococcus aureus was predicted,which would lay foundation on expression and evaluation of the major antigen epitope for immune efficacy." @default.
- W2348236180 created "2016-06-24" @default.
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- W2348236180 date "2012-01-01" @default.
- W2348236180 modified "2023-09-23" @default.
- W2348236180 title "Prediction of B cell antigenic epitope for vaccine candidate SirH against Staphylococcus aureus" @default.
- W2348236180 hasPublicationYear "2012" @default.
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