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- W2348238268 abstract "In order to acquire higher taxol-producing genetic-modified engineering isolates,the influence factors for protoplast preparation and regeneration of Pestalotiopsis microspora isolate PM-1 including the fungal age,enzyme system,permeability-stability agent,the time and temperature of enzymolysis,and the pH of initial medium were studied,based on explicating its taxol-producing ability.One mature method and system would be established for obtaining pure and high-yield protoplast by mixed-enzyme hydrolysis.It was suggested that many factors could affect the preparation and regeneration of P.microspora PM-1.Among above tested factors,the concerntration and time of enzymatic hydrolysis were the major ones.The experimental results showed that the most suitable procedures of protoplast preparation from Pestalotiopsis microspora isolate PM-1 were as follows: the conidia suspension should be cultured in Fries medium with pH 5.5 for 18 hours,the concentration of each enzyme including lywallzyme,drislase and snailase were all at 7.5 g/L,digestion was carried out for 3 hours at 36 ℃ and 0.7 mol/L NaCl was a suitable osmotic stabilizer.The results laid a foundation for the genetic improvement of strains and improving the rate of taxol produce." @default.
- W2348238268 created "2016-06-24" @default.
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- W2348238268 date "2013-01-01" @default.
- W2348238268 modified "2023-10-01" @default.
- W2348238268 title "The influence factors for protoplast preparation of a taxol-producing fungus PM-1 strain" @default.
- W2348238268 hasPublicationYear "2013" @default.
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