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- W2348250521 abstract "Objective To investigate the characteristics of the erythroid differentiation-related factor(EDRF) promoter,different lengths of EDRF promoters were cloned to drive GFP(green fluorescence protein,GFP) expression.Methods Different lengths of EDRF promoters(697,496,484,372 and 283 bp)were amplified by PCR.Then,the promoters were cloned into pcDNA-GFP vectors to drive GFP expression in vitro.After NIH3T3 and MEL cells were treated with the vectors,the activation of the promoter was analyzed by microscopy and FACS analysis.Results The 372 bp promoter(-116-+256 bp) was found to be the most effective one to drive GFP expression in NIH3T3 and Mel cells under microscopy.Moreover,the GFP positive rate of the 372 bp promoter group in Mel cells was obviously higher than that of other promoter groups by flow cytometry analysis(P0.01).The GFP positive rate of the 372 bp promoter group in NIH3T3 cells was(31.0±0.7)%,higher than that of other promoter groups(P0.01).While GFP positive rates of other promoter groups in NIH3T3 cells were more than 20%,which demonstrated that gene expression driven by the EDRF promoter was not specific in NIH3T3 cells,but specific in Mel cells. Conclusions The 372 bp EDRF promoter(-116-+256 bp) was the more effective one to drive GFP expression,which could be further used tostudy gene therapy for anemia." @default.
- W2348250521 created "2016-06-24" @default.
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- W2348250521 date "2010-01-01" @default.
- W2348250521 modified "2023-09-24" @default.
- W2348250521 title "GFP expression driven by an effective EDRF promoter" @default.
- W2348250521 hasPublicationYear "2010" @default.
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