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- W2348345046 abstract "Objective: To investigate the effects of rhIFN-a, rhIL-2 and rhTNF-a on the Hela cell line in vitro in a single or cooperative form or in combination with chemotherapeutics DDP.Methods: Chemosensitivity of the cells to doxorubicin was analysed using the MTT assay 12h and 24h after using rhIFN-a,rhIL-2 and rhTNF-a alone and in combination with chemotherapeutics DDP,and compared by using typanlan blue method.Results: When the three factors were used alone,IFN-a or TNF-a there had toxic action on the uterine cervix cancer Hela cell line,and when they were used cooperatively,there was significant difference in the effects on the Hela cell line(P0.01).All the cytokines had the enhancement of DDP antitumor activity in vitro with the dependence of the antitumor effect on their action order and time.Conclusion: The MTT assay is very rapid and simple.Chemosensitivity spectra vary with tumor types.rhIFN-a,rhIL-2 and rhTNF-a have the synergistic inhibition in combination with DDP,which provides provide better chemotherapy choice for patients with uterine cervix cancer." @default.
- W2348345046 created "2016-06-24" @default.
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- W2348345046 date "2005-01-01" @default.
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- W2348345046 title "In vitro synergistic inhibition of Hela cell by RhIFN-a、rhIL-2 and rhTNF-a" @default.
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