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- W2348349700 abstract "Objective To modify the techniques for primary culture of highly purified the primary rat alveolar macrophages(AMs).Methods AMs were isolated in vivo and cultured with such modified techniques as pre-injection of air and reclamation of brochoalveolar lavage fluid with 14-bugle syringe needle,low-speed centrifugation and multi-blowing before plating on 6-well culture plates.The morphological changes were observed under the inverted microscope.The cell survival rate was detected with trypan blue dye exclusion test,and cell purity was determined by Wright-Giemsa staining.Results In vitro,AMs adhered to plastic surface within 40 min;2-3 days later,cell morphology presented mixed shapes,i.e.,circular,irregular and spindle shapes.The trypan blue test showed a survival rate ≥95% of the AMs and Wright-Giemsa staining indicated a purity ≥95% of the AMs.Conclusion The modified techniques employed in our experiment had better results for the isolation and primary culture of AMs." @default.
- W2348349700 created "2016-06-24" @default.
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- W2348349700 date "2010-01-01" @default.
- W2348349700 modified "2023-09-23" @default.
- W2348349700 title "Modifications of the primary culture technique for rat alveolar macrophages" @default.
- W2348349700 hasPublicationYear "2010" @default.
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