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- W2348375025 abstract "To explore physiology-function difference of porcine antral granulosa cells(pMGCs)for subsequent toxicology research.pGCs was extracted from three antral follicles with different diameters(small follicles:2mm,middle follicles:2~5mm,large follicles:≥5mm)by mechanical separation,respectively,Cell viability was determined by trypan blue,cell purity was calculated by cell morphology under the invert microscope,gene expressions of FSHRand LHR were detected by real-time PCR to further identify pGCs,and growth curve of pGCs was detected by MTT assay.The results showed that pGCs viabilities were 68%,75%,72% and purities were 97%,95%,90%,respectively;growth curve showed that GCs entered the logarithmic growth phase after 24 hand reached the highest density on the 120 h,and growth behavior of pGCs from the middle follicles tumed to be the best;Moreover,real-time PCR assertained the above conclusions." @default.
- W2348375025 created "2016-06-24" @default.
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- W2348375025 date "2014-01-01" @default.
- W2348375025 modified "2023-09-25" @default.
- W2348375025 title "Comparison of Three Porcine Follicles Granulosa Cells with Different Diameters" @default.
- W2348375025 hasPublicationYear "2014" @default.
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