Matches in SemOpenAlex for { <https://semopenalex.org/work/W2348413628> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W2348413628 abstract "A nested PCR method was developed and validated for detection of Mycoplasma bovis in milk samples preserved with bronopol. A previously described, randomly cloned DNA fragment was sequenced. This sequence information was used to develop a set of internal primers for a nested PCR assay. Cationic surfactant purification was used to eliminate milk protein substances that inhibit amplification. Serial diluted seeded milk samples were used to determine a sensitivity of 5.1 CFU equivalents/ml of milk. Specificity of the assay was confirmed testing against 7 other mycoplasma species and 11 other mastitis organisms. A comparison of culture, with blind passage, to the nested PCR reaction was performed on clinical field milk samples from M. bovis affected and M. bovis free herds. The nested PCR method was more sensitive than culture (~5 CFU/ml), specific for M. bovis, effective with preserved milk, and could be completed in less than one day. This may provide a practical, rapid, cost effective procedure to screen for clinical and subclinical M. bovis carriers using routinely obtained preservative-treated milk samples." @default.
- W2348413628 created "2016-06-24" @default.
- W2348413628 creator A5017578870 @default.
- W2348413628 creator A5020797836 @default.
- W2348413628 creator A5021855051 @default.
- W2348413628 creator A5037821469 @default.
- W2348413628 date "2004-01-01" @default.
- W2348413628 modified "2023-09-26" @default.
- W2348413628 title "Development of a Nested PCR Method for Detection of Mycoplasma Bovis in Preserved Milk Samples" @default.
- W2348413628 cites W1964623895 @default.
- W2348413628 doi "https://doi.org/10.31274/ans_air-180814-847" @default.
- W2348413628 hasPublicationYear "2004" @default.
- W2348413628 type Work @default.
- W2348413628 sameAs 2348413628 @default.
- W2348413628 citedByCount "0" @default.
- W2348413628 crossrefType "report" @default.
- W2348413628 hasAuthorship W2348413628A5017578870 @default.
- W2348413628 hasAuthorship W2348413628A5020797836 @default.
- W2348413628 hasAuthorship W2348413628A5021855051 @default.
- W2348413628 hasAuthorship W2348413628A5037821469 @default.
- W2348413628 hasBestOaLocation W23484136281 @default.
- W2348413628 hasConcept C104317684 @default.
- W2348413628 hasConcept C140793950 @default.
- W2348413628 hasConcept C153911025 @default.
- W2348413628 hasConcept C157431215 @default.
- W2348413628 hasConcept C194775826 @default.
- W2348413628 hasConcept C2778838687 @default.
- W2348413628 hasConcept C49105822 @default.
- W2348413628 hasConcept C55493867 @default.
- W2348413628 hasConcept C86803240 @default.
- W2348413628 hasConcept C89423630 @default.
- W2348413628 hasConceptScore W2348413628C104317684 @default.
- W2348413628 hasConceptScore W2348413628C140793950 @default.
- W2348413628 hasConceptScore W2348413628C153911025 @default.
- W2348413628 hasConceptScore W2348413628C157431215 @default.
- W2348413628 hasConceptScore W2348413628C194775826 @default.
- W2348413628 hasConceptScore W2348413628C2778838687 @default.
- W2348413628 hasConceptScore W2348413628C49105822 @default.
- W2348413628 hasConceptScore W2348413628C55493867 @default.
- W2348413628 hasConceptScore W2348413628C86803240 @default.
- W2348413628 hasConceptScore W2348413628C89423630 @default.
- W2348413628 hasLocation W23484136281 @default.
- W2348413628 hasLocation W23484136282 @default.
- W2348413628 hasOpenAccess W2348413628 @default.
- W2348413628 hasPrimaryLocation W23484136281 @default.
- W2348413628 hasRelatedWork W1836626573 @default.
- W2348413628 hasRelatedWork W1898295043 @default.
- W2348413628 hasRelatedWork W2061552919 @default.
- W2348413628 hasRelatedWork W2088661384 @default.
- W2348413628 hasRelatedWork W2137641501 @default.
- W2348413628 hasRelatedWork W2350388776 @default.
- W2348413628 hasRelatedWork W2480411 @default.
- W2348413628 hasRelatedWork W2758964714 @default.
- W2348413628 hasRelatedWork W2784145329 @default.
- W2348413628 hasRelatedWork W3153631585 @default.
- W2348413628 isParatext "false" @default.
- W2348413628 isRetracted "false" @default.
- W2348413628 magId "2348413628" @default.
- W2348413628 workType "report" @default.