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- W2348440492 abstract "Poplar embryonic calli were treated with ethyl methanesulfonate(EMS) to get the salt-tolerant mutants through tissue culture and directional selection.The research into EMS concentration and treatment time showed that at a given concentration,the buds differentiated from the calli reduced with the treatment time increasing,and at a given treatment time,the buds differentiated also reduced with the concentration increasing.There was a linear relationship between the treatment time and the differentiation rate under different EMS concentrations.About 2 million embryonic cells treated with EMS at a concentration of 4‰(V/V) for 36 h could produce 40 tissue-cultured rooted plantlets on the medium containing 0.5% NaCl through step-by-step directional selection of salt tolerance.The test for the salt tolerance of plantlets showed that the plantlets obtained through EMS treatment had a higher salt tolerance than those obtained without EMS treatment." @default.
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- W2348440492 date "2007-01-01" @default.
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- W2348440492 title "Selection of salt-tolerant mutants from ethyl methanesulfonate-mutagenized poplar embryonic calli" @default.
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