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- W2348486421 abstract "Objective:To construct the recombinant plasmid pcDNA3.1-ANXA5,transfect it into uterine cervical carcinoma cell line and detect its expression.Methods:Annexin A5(ANXA5)gene containing BamHI and XhoI endoenzyme sites was obtained by PCR;Double enzyme digestion with BamHI and XhoI was con- ducted for pcDNA3.1 plasmid.The pcDNA3.1 plasmid and ANXA5 gene with the same endings were linked by T4 lingase.After the ANXA5 gene in the recombinant plasmid pcDNA3.1-ANXA5 was detected correct,the recombinant plasmid was transfected into uterine cervical carcinoma cell line SiHa cells and the overexpression of ANXA5 protein was detected by Western blot.Results:The sequence of ANXA5 gene in the recombinant plasmid was proved correct and after being transfected into SiHa cells,the ANXA5 protein was overexpressed. Conclusions:The recombinant plasmid pcDNA3.1-ANXA5 is constructed successfully and the SiHa cells trans- fected with the plasmid can overexpress ANXA5 protein,which may facilitate the study on ANXA5 function in tumor cells." @default.
- W2348486421 created "2016-06-24" @default.
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- W2348486421 date "2008-01-01" @default.
- W2348486421 modified "2023-09-24" @default.
- W2348486421 title "CONSTRUCTION OF RECOMBINANT PLASMID PCDNA3.1-ANXA5 AND ITS TRANSFECTION AND OVEREXPRESSION IN SIHA CELLS" @default.
- W2348486421 hasPublicationYear "2008" @default.
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