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- W2348526638 abstract "Aim:To observe the polymorphism of the mitochondrial cytochrome c-oxidase subunit Ⅰgene of different T. spralis isolates.Methods:PCR-single-strand conformation polymorphism (PCR-SSCP) was used to analyze the polymorphism of the partial mitochondrial cytochrome c-oxidase subunit Ⅰ gene (COXⅠ) of seven swine T.spiralis(T1) isolates from Henan, Yunnan, Harbin,Tongjiang in Heilongjiang, Hubei and Tianjin as well as one Poland isolate from domestic pig.Results: The results of electrophoresis of PCR products of Trichinella COXⅠ gene showed that the band (400 bp) of seven Trichinella isolates from China was the same as Poland isolate. Three genotypes (AA, AB and BB) among different Trichinella isolates were observed by SSCP. Poland isolate was AA genotype, Tianjin was BB genotype, other isolates were AB genotype. The frequency of AB genotype was the highest (0.75), the frequency of AA and BB genotype was 0.125, and the frequency of A allele was equal to B allele (0.5). The polymorphism information content (PIC) of different Trichinella isolates was 0.375.Conclusion: The polymorphism of the COXⅠ gene of eight swine Trichinella isolates was moderate." @default.
- W2348526638 created "2016-06-24" @default.
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- W2348526638 date "2009-01-01" @default.
- W2348526638 modified "2023-09-26" @default.
- W2348526638 title "PCR-SSCP analysis on partial mitochondrial cytochrome c-oxidase subunit I (COXI) genes of different Trichinella spiralis isolates" @default.
- W2348526638 hasPublicationYear "2009" @default.
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