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- W2348552521 abstract "Objective To develop a simple and reproductable method for the isolation and primary culture of pulmonary microvascular endothelial cells(PMVECs) of rats.Methods The tissue block pasted culture method was improved in experiment animal,culture-medium,tissue block method,fetal bovine serum(FBS) and spreading slide method.Rats PMVECs were primary cultured with lung tissue block pasted methods using lung tissue of male Wistar rats,and the cell morphology was observed using inverted microscope and electron microscope,and identified using immunohistochemical methods.Results The cultured PMVECs of rats in vitro showed fusiform shape or polygon,and the monolayer cultures displayed a typical cobblestone or pavingstone morphology and were inhibited when contacted each other,the PMVECs expressed factor Ⅷ associated antigen.And the primary culture of PMVEC was established successfully.Conclusion The pure pulmonary MVECs of rats can be attained rapidly and easily by the described method.The method is simple,economic and reliable,availability of these cells will facilitate further functional character studies." @default.
- W2348552521 created "2016-06-24" @default.
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- W2348552521 date "2007-01-01" @default.
- W2348552521 modified "2023-09-24" @default.
- W2348552521 title "Culture of Pulmonary Microvascular Endothelial Cells in Rats" @default.
- W2348552521 hasPublicationYear "2007" @default.
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