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- W2348568545 abstract "To obtain a set of probe which is high-sensitivity and high specificity,we structure work standard by genetic engineering and develop the method of fluorescence quota PCR for detected HBV-DNA.And using real-time PCR to detected HBV-DNA national standards.The Result demonstration,successful construction work standard and the linearity range of real-time PCR for detected HBV-DNA is 109copies/mL to 103copies/mL.The intra and inter Coefficients of variation were less than 1.44% and 5.63%,respectively.The positive rate and negative rate were 100% respectively,compared with the national positive/negative reference materials.The linear correlation coefficient of the national linear standard's examination value and theoretical value is 0.998.These results demonstrate that the assay was successful developed for detected HBV-DNA by real time PCR,which was high-sensitive and high-specific." @default.
- W2348568545 created "2016-06-24" @default.
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- W2348568545 date "2012-01-01" @default.
- W2348568545 modified "2023-09-26" @default.
- W2348568545 title "SCREENING OF HIGHLY EFFECTIVE PROBE FOR REAL-TIME PCR AND DEVELOPMENT OF DETECTED HEPATITIS BVIRUS DNA" @default.
- W2348568545 hasPublicationYear "2012" @default.
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