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- W2348621020 abstract "To establish a cell line expressing human MAdCAM-1 molecular.Methods: hMAdCAM-1 cONA was achieved from plasmid pUC21/hMAdCAM-1 by restriction enzyme digestion. Hie recombinant eukaryotic expression vector pCIneo-hMAd was constructed and then transfected into CHO cells with lipofectamine. Results: G418-resistant clones and their expression products were identified by immunofluo-rescence、immunoenzymatic histochemistry、 western blot and lymphocytes adhesion assay. hMAdCAM-1 with relative molecule mass of 63 000 could be demonstrated in cell lysates of the pCIneo-hMAd transfected cells. Conclusion: A stable transfectant expressing hMAdCAM-1 is successfully established, which might provide the basis for further studying the biologic functions and their mechanism." @default.
- W2348621020 created "2016-06-24" @default.
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- W2348621020 date "2002-01-01" @default.
- W2348621020 modified "2023-09-25" @default.
- W2348621020 title "Construction and expression of human MAdCAM-1 eukaryotic expression vector" @default.
- W2348621020 hasPublicationYear "2002" @default.
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