Matches in SemOpenAlex for { <https://semopenalex.org/work/W2348645232> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2348645232 abstract "The objective of the experiment is to explore the purification and production of immunotoxin .The chimeric toxin,which is composed of 40 peptides of interleukin 10(from amino acids 18 to 57) fused to a mutant form of Pseudomonas extoxin(PE) devoid of its native cell recognition domain.Two kinds of prokaryotic expression vector containing the chimeric toxin IL-10_ 18-57-PE40 were constructed respectively . After induction of IPTG for 3 hours,IL-10_ 18-57-PE40 was expressed highly in cytoplasmic fraction in Rosettablue(DE3),and was directed to periplasmic space as soluble form in E.coli BL21(DE3)pLysS .Western -blotting showed that the expressed protein could react with the specific rabbit sera against LHRH-PE40.With the application of salting out of (NH_4)_2SO_4, hydrophobic interaction chromatography,Cu-affinity chromatography and anion exchange chromatography ,the purity of IL-10_ 18-57-PE40 was about 96%.The cytotoxicity assay ,Cell-ELISA and fluorescent antibody test support the hypothesis that IL-10_ 18-57 based ligand-mediated cytotoxicity can serve to target cytotoxic agents in vitro." @default.
- W2348645232 created "2016-06-24" @default.
- W2348645232 creator A5009578246 @default.
- W2348645232 date "2006-01-01" @default.
- W2348645232 modified "2023-09-23" @default.
- W2348645232 title "High Level Expression,Purification and Cytotoxicity of IL-10_(18-57)-PE40" @default.
- W2348645232 hasPublicationYear "2006" @default.
- W2348645232 type Work @default.
- W2348645232 sameAs 2348645232 @default.
- W2348645232 citedByCount "0" @default.
- W2348645232 crossrefType "journal-article" @default.
- W2348645232 hasAuthorship W2348645232A5009578246 @default.
- W2348645232 hasConcept C104317684 @default.
- W2348645232 hasConcept C109316439 @default.
- W2348645232 hasConcept C115085202 @default.
- W2348645232 hasConcept C123894998 @default.
- W2348645232 hasConcept C153911025 @default.
- W2348645232 hasConcept C154317977 @default.
- W2348645232 hasConcept C179247698 @default.
- W2348645232 hasConcept C181199279 @default.
- W2348645232 hasConcept C185592680 @default.
- W2348645232 hasConcept C201663137 @default.
- W2348645232 hasConcept C202751555 @default.
- W2348645232 hasConcept C2777367657 @default.
- W2348645232 hasConcept C40767141 @default.
- W2348645232 hasConcept C547475151 @default.
- W2348645232 hasConcept C55493867 @default.
- W2348645232 hasConcept C86803240 @default.
- W2348645232 hasConceptScore W2348645232C104317684 @default.
- W2348645232 hasConceptScore W2348645232C109316439 @default.
- W2348645232 hasConceptScore W2348645232C115085202 @default.
- W2348645232 hasConceptScore W2348645232C123894998 @default.
- W2348645232 hasConceptScore W2348645232C153911025 @default.
- W2348645232 hasConceptScore W2348645232C154317977 @default.
- W2348645232 hasConceptScore W2348645232C179247698 @default.
- W2348645232 hasConceptScore W2348645232C181199279 @default.
- W2348645232 hasConceptScore W2348645232C185592680 @default.
- W2348645232 hasConceptScore W2348645232C201663137 @default.
- W2348645232 hasConceptScore W2348645232C202751555 @default.
- W2348645232 hasConceptScore W2348645232C2777367657 @default.
- W2348645232 hasConceptScore W2348645232C40767141 @default.
- W2348645232 hasConceptScore W2348645232C547475151 @default.
- W2348645232 hasConceptScore W2348645232C55493867 @default.
- W2348645232 hasConceptScore W2348645232C86803240 @default.
- W2348645232 hasLocation W23486452321 @default.
- W2348645232 hasOpenAccess W2348645232 @default.
- W2348645232 hasPrimaryLocation W23486452321 @default.
- W2348645232 hasRelatedWork W113434161 @default.
- W2348645232 hasRelatedWork W1494165537 @default.
- W2348645232 hasRelatedWork W1568072008 @default.
- W2348645232 hasRelatedWork W1969565497 @default.
- W2348645232 hasRelatedWork W1970076404 @default.
- W2348645232 hasRelatedWork W2024985136 @default.
- W2348645232 hasRelatedWork W2064362510 @default.
- W2348645232 hasRelatedWork W2348834142 @default.
- W2348645232 hasRelatedWork W2355931576 @default.
- W2348645232 hasRelatedWork W2364123382 @default.
- W2348645232 hasRelatedWork W2364443566 @default.
- W2348645232 hasRelatedWork W2366137766 @default.
- W2348645232 hasRelatedWork W2369132793 @default.
- W2348645232 hasRelatedWork W2377322754 @default.
- W2348645232 hasRelatedWork W2379090347 @default.
- W2348645232 hasRelatedWork W2380715748 @default.
- W2348645232 hasRelatedWork W2390557301 @default.
- W2348645232 hasRelatedWork W2391325305 @default.
- W2348645232 hasRelatedWork W2391504631 @default.
- W2348645232 hasRelatedWork W2467036629 @default.
- W2348645232 isParatext "false" @default.
- W2348645232 isRetracted "false" @default.
- W2348645232 magId "2348645232" @default.
- W2348645232 workType "article" @default.