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- W2348651131 abstract "HCV/E2 gene fragment was obtained from plasmid pUC18/HCV E2 by digesting with endonuclease BamHI,another E2 fragment was obtained from same pladmid by digesting with NcoⅠ and HindⅢ.Two fragments were inserted into proper reading frame of expression vector pRSETHis respectively,and recombiant expression plasmids of pRSET·C/E2 and pRSET·A/E2were constructed.these recombinant plasmids were transformed into E.coli.TOP10 strain and induced by IPTG.The expressive conditions were optimized,the induced purpose protein was detected and analysed by SDS PAGE and Western blot.The pRSET·A/E2 have a high level expression and the E2 fusion protein accounted for 20% in the total proteins of host bacteria meanwhile the pRSET·C/E2 have not detected expressive purpose protein entirely.We guess the reason of that is due to a very stable RNA secondary strcucture within mRNA translation initial region in pRSET·C/E2." @default.
- W2348651131 created "2016-06-24" @default.
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- W2348651131 date "2003-01-01" @default.
- W2348651131 modified "2023-09-23" @default.
- W2348651131 title "Effect of gene structure of recombine plasmid in HCV/E2 protein Expression" @default.
- W2348651131 hasPublicationYear "2003" @default.
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