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- W2348657490 abstract "To construct a recombinant plasmid expressing the abhesin gene HpaA from clinical strains of Heliobacter pylori(Hp),to express the recombinant proteins in E.coli,and to determine its diagnostic value,the gene encoding the adhesion subunit protein was cloned, sequenccd and expressed.The DNA fragment to the HapA gene from clinical strains of Hp was amplificed by PCR.After cloning and sequence analysis,it was expressed with high efficency in E.coli,and the expressed products after purification and identification with Western blotting could be used as the Hp antigen.The feasibility of its clinical applications with comparative evaluation was done by the antibody testing with ELISA,germ-cultures,histological staining and rapid urease test in 100 clinical specimens.It was found that the total length of gene cloned was 783 bp with a 98.8% sequence homology with adhesin genes in GenBank,and the molecular weight was found to be 30kDa as determined by SDS-PAGE.The percentage of soluble expression was about 41.67% of total cell proteins.After purification with affinity chromatography the purity of the recombinant protein was about 90%,and its immumnogenicity was confirmed by Western blotting.As judging with 100 clinical specimens,it was also found that the percentages of germ-culture,testing,histology staining test,rapid urease test,and the sensitivity,specificity and accuracy of HpaA antibody assay were 100%,80.9%,100%,90.5%,85.8%,93.3%,and 85.9% respectively.It conculudes that rHpaA is well expressed in E.coli and the hpaA protein has perfect immunogenicity." @default.
- W2348657490 created "2016-06-24" @default.
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- W2348657490 date "2004-01-01" @default.
- W2348657490 modified "2023-09-28" @default.
- W2348657490 title "Cloning and expression of the adhesin gene HpaA from clinical strains of Helicobacter pylori and its diagnostic value" @default.
- W2348657490 hasPublicationYear "2004" @default.
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