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- W2348657512 abstract "Objective:To establish the method of isolation and culture of dendritic cells derived from exudative malignant pleural effusions (EMPE) of non-small cell lung cancer, to study the regularity by which PEDCs express functional antigens. Methods: Using the method of density gradient centrifugation, we obtained one groups of cells from PEDC-abundant EMPE in NSCLC, and purified the PEDCs, using CD3 immunomagnetic beads. Then we cultured PEDCs using IL-4, GM-CSF and TNF-α. When PEDCs had been cultured for 1d, 2d, 4d and 8d, the functional antigens of PEDCs were analysed by flow cytometry. Results:The method for isolation and culturc of PEDCs was established. The regularity was demonstrated that PEDCs cultured for 2d expressed HLA-ABC, HLA-DR, CD80, CD83, CD1a, and CD54 at the highest magnitude. Conclusions: PEDCs isolated from EMPE and cultured for 2d in vitro, expressed many functional antigens at the highest magnitude." @default.
- W2348657512 created "2016-06-24" @default.
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- W2348657512 date "2004-01-01" @default.
- W2348657512 modified "2023-09-25" @default.
- W2348657512 title "The Isolation, Culture and Identification of Dendritic Cells Derived from EMPE in NSCLC" @default.
- W2348657512 hasPublicationYear "2004" @default.
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