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- W2348657574 abstract "[ Objective] To isolate and identify astrocytes from cerebral cortex of neonatal rats. [Methods] The cells were isolated from cerebral cortex of neonatal rats by using MEM media added with 10% equine serum , 10% bovine serum and 10 ng/mL EGF; then the cells were fixed in the third to fourth week after culture and immunocytochemistry was used to detect specific GFAP antigen of the cells. [Result] The cells isolated from cerebral cortex of neonatal rats grew well and expressed GFAP antigen. [Conclusion] We established a simple, reliable method of culturing and purifying astrocytes of neonatal rats and underlined the co-culturing astrocyte feeder layer with other cells culture in the future." @default.
- W2348657574 created "2016-06-24" @default.
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- W2348657574 date "2005-01-01" @default.
- W2348657574 modified "2023-09-25" @default.
- W2348657574 title "Isolation and identification of astrocytes from cerebral cortex of neonatal rats" @default.
- W2348657574 hasPublicationYear "2005" @default.
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