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- W2348658634 abstract "Objective To investigate the permeability changes of prostate cancer cells membrane under low intensity ultrasound in vitro. Methods The culture of monolayer adherent LNCaP prostate cancer cells in six-well plate was exposed to continuous ultrasound at frequency of 1 MHz. The cells membrane permeability (stained with Calcein)and cells viability(stained with PI)were evaluated by fluorescent microscope (FM) and cells morphological changes were evaluated by scanning electron microscope (SEM) under ultrasound with acoustic intensity of 160 mW/cm2 for 5 s. The rate of cells with increased cells membrane permeability as function of acoustic intensity (80 mW/cm2,120 mW/cm2 and 160 mW/cm2,for 5 s) and exposure duration (5 s,10 s and 15 s,acoustic intensity of 120 mW/cm2) was evaluated by flow cytometry. Results After low intensity of ultrasound,the cells with increased cell membrane permeability could be clearly shown with Calcein uptake under FM while no cell showed Calcein uptake in the control group. The SEM showed less microvilli on the cells after low intensity of ultrasound exposure and few cells showed holes on the cell membrane. The rate of cells with increased membrane permeability increased with acoustic intensity and exposure duration. Conclusion Low intensity ultrasound alone could increase membrane permeability of prostate cancer cells and cells with increased membrane permeability showed surface plane,uncommon holes on the cells membrane. The rate of cells with increased membrane permeability positively correlated with acoustic intensity and exposure duration." @default.
- W2348658634 created "2016-06-24" @default.
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- W2348658634 date "2010-01-01" @default.
- W2348658634 modified "2023-09-23" @default.
- W2348658634 title "Permeability increase of prostate cancer cells membrane under low intensity ultrasound in vitro" @default.
- W2348658634 hasPublicationYear "2010" @default.
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