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- W2348662010 abstract "Objective To survey the effects of advanced glycosylation end products (AGEs) on proliferation and differentiation of neural precursor cells (NPCs) from embryonic rat hippocampus. Methods Single cell suspension was prepared from the hippocampus of 14.5 days embryos rat. After formation of the primary neurospheres,single cell clone was obtained with limited dilution method.Then the single cell clone spheres were digested and induced to proliferate and differentiate using non-serum medium and bovine serum.The effects of varied concentrations of AGE-BSA on the proliferation and differentiation of NPCs were investigated by mononuclear cell direct cytotoxity (MTT) reduction assay and immumofluorescence laser scanning method,respectively.Results AGEs-BSA (50-400 mg/L) could decrease the absorbance (A570 nm) on days 3 and 7 after intervention significantly(P0.01). Percentage of MAP2 positive cells was significantly lower after 7-day intervention of AGEs-BSA (50-400 mg/L) than that in group C (P0.05).Conclusion AGEs inhibit the proliferation and differentiation of NPCs from embryonic rat hippocampus." @default.
- W2348662010 created "2016-06-24" @default.
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- W2348662010 date "2009-01-01" @default.
- W2348662010 modified "2023-09-23" @default.
- W2348662010 title "Effects of advanced glycation end products on proliferation and differentiation of neural precursor cells from embryonic rat hippocampus" @default.
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