Matches in SemOpenAlex for { <https://semopenalex.org/work/W2348666956> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2348666956 abstract "Objective To determine the concentration of protein in lipid samples by the ethanol precipitation-BCA method.Methods Three-phase diagram for bovine serum albumin(BSA)-water-ethanol mixture and the effect of ethanol/lipid(V/V)on turbidity of lipid were investigated to determine the voluminal ratio of ethanol to sample,when ethanol was used to precipitate protein.Cold ethanol centrifugation was adoped to precipitate protein and the 2,2'-biquinoline-4,4-dicarboxylic acid disodium salt(BCA)method was used to determine concentration of protein.Results Using ethanol to precipitate protein before protein determination by BCA eliminated lipid effect.The good linear relation was showed as BSA being ranged within 100-1 000 μg·mL-1(r=0.998 9),the relative standard deviation(RSD,%)and recoveries were all consistent with requirement of analysis.Conclusion The protein concentration in lipid samples can be easily and accurately determined by ethanol precipitation-BCA method,which avoiding interference of lipids." @default.
- W2348666956 created "2016-06-24" @default.
- W2348666956 creator A5082703961 @default.
- W2348666956 date "2010-01-01" @default.
- W2348666956 modified "2023-09-27" @default.
- W2348666956 title "Determination of Protein in Lipid Samples by the Ethanol Precipitation-BCA Method" @default.
- W2348666956 hasPublicationYear "2010" @default.
- W2348666956 type Work @default.
- W2348666956 sameAs 2348666956 @default.
- W2348666956 citedByCount "0" @default.
- W2348666956 crossrefType "journal-article" @default.
- W2348666956 hasAuthorship W2348666956A5082703961 @default.
- W2348666956 hasConcept C107054158 @default.
- W2348666956 hasConcept C121332964 @default.
- W2348666956 hasConcept C153294291 @default.
- W2348666956 hasConcept C185592680 @default.
- W2348666956 hasConcept C18903297 @default.
- W2348666956 hasConcept C2780161600 @default.
- W2348666956 hasConcept C43617362 @default.
- W2348666956 hasConcept C4725764 @default.
- W2348666956 hasConcept C53941386 @default.
- W2348666956 hasConcept C55493867 @default.
- W2348666956 hasConcept C64016661 @default.
- W2348666956 hasConcept C74774607 @default.
- W2348666956 hasConcept C7623868 @default.
- W2348666956 hasConcept C86803240 @default.
- W2348666956 hasConcept C86807702 @default.
- W2348666956 hasConceptScore W2348666956C107054158 @default.
- W2348666956 hasConceptScore W2348666956C121332964 @default.
- W2348666956 hasConceptScore W2348666956C153294291 @default.
- W2348666956 hasConceptScore W2348666956C185592680 @default.
- W2348666956 hasConceptScore W2348666956C18903297 @default.
- W2348666956 hasConceptScore W2348666956C2780161600 @default.
- W2348666956 hasConceptScore W2348666956C43617362 @default.
- W2348666956 hasConceptScore W2348666956C4725764 @default.
- W2348666956 hasConceptScore W2348666956C53941386 @default.
- W2348666956 hasConceptScore W2348666956C55493867 @default.
- W2348666956 hasConceptScore W2348666956C64016661 @default.
- W2348666956 hasConceptScore W2348666956C74774607 @default.
- W2348666956 hasConceptScore W2348666956C7623868 @default.
- W2348666956 hasConceptScore W2348666956C86803240 @default.
- W2348666956 hasConceptScore W2348666956C86807702 @default.
- W2348666956 hasLocation W23486669561 @default.
- W2348666956 hasOpenAccess W2348666956 @default.
- W2348666956 hasPrimaryLocation W23486669561 @default.
- W2348666956 hasRelatedWork W1966425291 @default.
- W2348666956 hasRelatedWork W2014392744 @default.
- W2348666956 hasRelatedWork W2020460058 @default.
- W2348666956 hasRelatedWork W2035542393 @default.
- W2348666956 hasRelatedWork W2042003881 @default.
- W2348666956 hasRelatedWork W2045666653 @default.
- W2348666956 hasRelatedWork W2065073955 @default.
- W2348666956 hasRelatedWork W2083205453 @default.
- W2348666956 hasRelatedWork W2091681674 @default.
- W2348666956 hasRelatedWork W2102312996 @default.
- W2348666956 hasRelatedWork W2148117412 @default.
- W2348666956 hasRelatedWork W2149453380 @default.
- W2348666956 hasRelatedWork W2364304374 @default.
- W2348666956 hasRelatedWork W2366962791 @default.
- W2348666956 hasRelatedWork W2367070627 @default.
- W2348666956 hasRelatedWork W2378364511 @default.
- W2348666956 hasRelatedWork W2390317397 @default.
- W2348666956 hasRelatedWork W2390466658 @default.
- W2348666956 hasRelatedWork W2392784691 @default.
- W2348666956 hasRelatedWork W423616459 @default.
- W2348666956 isParatext "false" @default.
- W2348666956 isRetracted "false" @default.
- W2348666956 magId "2348666956" @default.
- W2348666956 workType "article" @default.