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- W2348682912 abstract "Aim To construct HPV16 E5 clone and prepare for advanced research. Methods The E5 fragment of HPV16 was obtaind by PCR and cloned to pGEM-T. The DNA fragment digested from the pGEM-T /HPV16 E5 with BamHⅠand EcoRⅠwas ligated to the BamHⅠ-EcoRⅠ-digested pGEX-2T prokaryotic expressing vectors. The expression of fusion protein was induced by IPTG and the product was applied to SDS-PAGE. Results 1The sequencing , PCR and endonucleases digestion results showed that the E5 fragment was insert into pGEM-T and PGEX-2T vectors. 2SDS-PAGE showed the HPV16 E5 gene has been expressed in DH5αcells. Conclusion Successfully construct the prokaryotic recombinant plasmid of HPV16 E5 and the fusion protein can be used as immunogens to generate antibodies to detect native viral proteins." @default.
- W2348682912 created "2016-06-24" @default.
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- W2348682912 date "2001-01-01" @default.
- W2348682912 modified "2023-09-23" @default.
- W2348682912 title "Cloning and prokaryotic expressing of human papillomavirus type 16 E5 transforming gene" @default.
- W2348682912 hasPublicationYear "2001" @default.
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