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- W2348695068 abstract "Objective:To transfect the Dendritic cells (DC) with recombinant adenovirus vector AdmIL-12,make vaccine of DC.Methods:DC was transfected by AdmIL-12 and its phenotype was measured with flowcytometery. The expression of IL-12 in DC was valued with PT-PCR method. The value of IL-12 and IFN-γ in culture supernatants was nanlyzed by ELISA. The biological activity of IL-12 secreted from DC was tested by its ability to stimulate T cells to secret IFN-γ. Results: Recombinant adenovirus AdmIL-12 transfected DC effectively. After transfetion,the phenotype of DC had no significant changement,IL-12 was expressed more in DC,DC secreted more IL-12 with biological activity. Conclusion:AdmIL-12 transfected DC effectively. The vaccine of DC was made successfully." @default.
- W2348695068 created "2016-06-24" @default.
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- W2348695068 date "2009-01-01" @default.
- W2348695068 modified "2023-09-28" @default.
- W2348695068 title "Gene Transfection of Dendritic Cells by Recombinant Adenovirus AdmIL-12" @default.
- W2348695068 hasPublicationYear "2009" @default.
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