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- W2348771007 abstract "To develop a HPLC - Ion trap mass spectrometry method for qualitative and quantitative analysis of penicillin G and cefradine in human plasma. The drug in plasma was extracted by solid phase extraction column and detected by HPLC - Ion trap mass spectrometry. The liquid chromatography system was equipped with Merck - RP C18 column. The mobile phase consisted of methanol - water - acetic acid (25:75:0.01, by volume), with a flow rate of 0.3 mL/min. Electrospray ionization(ESI) source was applied and operated in the positive ion mode. The qualitative analysis using a combination of selected ion monitoring (SIM) and full scan MS - MS. The daughter ion of m/z 159 and m/z 289 were used to quantify cefradine and penicillin G. The linear concentration range of the standard curves for cefradine and penicillin G were both 20-1 000 ng/mL. The LOD of cefradine and penicillin G were 5 ng/mL(S/N 20) and 1 ng/mL(S/N 15). The method was simple, specific and sensitive for qualitative and quantitative analysis of Penicillin G and Cefradine in human plasma." @default.
- W2348771007 created "2016-06-24" @default.
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- W2348771007 date "2004-01-01" @default.
- W2348771007 modified "2023-09-24" @default.
- W2348771007 title "Determination of Penicillin G and Cefradine in the Human Plasma by HPLC - Ion Trap Mass Spectrometry" @default.
- W2348771007 hasPublicationYear "2004" @default.
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