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- W2348771488 abstract "AIM: To explore the transcription regulation of 5Aza-2'-deoxycytidine(5-Aza-CdR) on RIZ1 tumor suppressor gene in human gastric carcinoma BGC823 cell line and to investigate its effect on the growth of BGC823 cells.METHODS: Human gastric carcinoma BGC823 cells were treated with different doses of 5-Aza-CdR,the status of 5'CpG island methylation of RIZ1 gene in BGC823 cells was analyzed using methylation specific polymerase chain reaction(MSP),and reverse transcription polymerase chain reaction(RT-PCR) was used to examine the expression of RIZ1 gene in BGC823 cells.Proliferations of BGC823 cells treated by 5-Aza-CdR were observed by MTT assay.The cell cycle and apoptosis was analyzed by flow cytometry.RESULTS: Before 5-Aza-CdR treatment,promoter hypermethylation of RIZ1 gene existed and the RIZ1 mRNA was not expressed in BGC823 cells,after 2,5,10 μmol/L 5-Aza-CdR treatment for 48 h,hypermethylated RIZ1 gene was demethylated and RIZ1 mRNA was expressed.Administration with different doses of 5-Aza-CdR,BGC823 cells displayed a slowed proliferations rate in contrast to the control group(P0.05),and its inhibitory effect showed a dose-dependent manner.Flow cytometry showed that 5-Aza-CdR significantly increased the cell number in G0/G1 phase,decreased the cell number in S phase and induced the apoptosis of BGC823 cells compared with the control group(P0.05).CONCLUSION: 5-Aza-CdR may effectively cause the demethylation of RIZ1 gene CpG-rich promoter regions,activate its expression,and inhibit the growth of BGC823 cells." @default.
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- W2348771488 date "2008-01-01" @default.
- W2348771488 modified "2023-09-24" @default.
- W2348771488 title "Transcription regulation of 5-Aza-2'-deoxycytidine on RIZ1 gene demethylation in human gastric carcinoma BGC823 cell line" @default.
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