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- W2348790091 abstract "Using hypocotyl segments obtained from sterilized bud of A.membranaceus var.mongholicus and root,stem and leaf from sterilized plants as explants,then germinating explants on MS medium supplemented with different combinations and concentrations of hormone.The temperature was controlled(25±1)℃ under dark conditions,the suger was 3.0%,pH was 5.8 and agar was 0.8%.To induce callus of A.membranaceus var.mongholicus,the maximum and the best callus were achived on MS medium with 2.0 mg/L 2,4-D and 0.5 mg/L 6-BA,and the other culture could receive the same yield of callus wad MS medium supplamented with 3.0 mg/L NAA and 0.5 mg/L 6-BA.On both medium and the explants could initate 100%.The optimal explant was leaf and the medium was MS+NAA 3.0 mg/L.We chose MS+NAA 3.0 mg/L+6-BA 0.5 mg/L as callus subculture and hypocotyl segmants as explant.Callus growth curve of A.membranaceus var.mongholicus showd S.The time of logarithim growth appeared after being cultured for 9 days,we confirmed that about 24 days were the time of exchange medium." @default.
- W2348790091 created "2016-06-24" @default.
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- W2348790091 date "2008-01-01" @default.
- W2348790091 modified "2023-09-28" @default.
- W2348790091 title "Callus Initiation of Astragalus membranaceus var.mongholicus" @default.
- W2348790091 hasPublicationYear "2008" @default.
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