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- W2348823265 abstract "Objective Avian influenza virus (AIV) hemagglutinin (H5N1) gene was transformed into the Lotus corniculatus. Legume forage was used as a plant bioreactor to produce the H5N1 antigen protein in order to investigate the feasibility of developing edible transgenic plant vaccine of AIV.MethodTaking the cotyledon petiole explant as the transformation receptors,hemagglutinin gene of avian influenza was transformed into Lotus corniculatus by Agrobacterium-mediated transformation. After co-culture,selective differentiation and regeneration,resistant plants were obtained. Resistant plants were detected by PCR,RT-PCR and western blotting.ResultThe results have proved that hemagglutinin gene has been transformed into the genome of Lotus corniculatus. The hemagglutinin gene has been expressed on protein level and nucleic acid level in the transgenic plants and the result is better than the control.ConclusionIt is feasible to use plant bioreactor to produce AIV H5N1 antigen protein in the legume forage." @default.
- W2348823265 created "2016-06-24" @default.
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- W2348823265 date "2008-01-01" @default.
- W2348823265 modified "2023-09-25" @default.
- W2348823265 title "Study on Expression of Avian Influenza Virus Hemagglutinin (H5N1) Gene in Lotus corniculatus" @default.
- W2348823265 hasPublicationYear "2008" @default.
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