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- W2348824726 abstract "It was reported previously that a 31 bp DNA fragment located at 5' upstream region of rice Wx gene could bind the nuclear proteins extracted from immature seeds of rice plant. To analyze the function of this 31 bp fragment, a series of Wx gene promoter regions with or without the 31 bp fragment were ligated with the coding sequence of GUS gene to construct chimeric plasmids(Fig.1). These constructs were inserted in Ti plasmids and introduced into immature embrogenic calli of rice through Agrobacterium mediated gene transfer system, and a number of transgenic plants were regenerated. The GUS activities in the transformed calli(Fig.2) and the immature seeds of transgenic plants(Fig.3) were determined. The data show that the 31 bp fragment enhances GUS gene expression 2～3 times as high as that of the Wx gene promoter region not including it." @default.
- W2348824726 created "2016-06-24" @default.
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- W2348824726 date "2000-01-01" @default.
- W2348824726 modified "2023-09-23" @default.
- W2348824726 title "A 31 bp Fragment Within the 5' Upstream Region of Rice Waxy Gene Enhances Gene Expression" @default.
- W2348824726 hasPublicationYear "2000" @default.
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