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- W2348835848 abstract "Objective To establish a molecular biological method for detection of legionella species.Methods Routine Polymerase Chain Reaction(PCR) was used to amplify the specific fragment of legionella species 16SrRNA gene and lepionella pneumophila(Lp) mip gene while semi-nested PCR was used to validate the amplified product of routine PCR.Results The 654bp specific fragment of 16SrRNA gene of 5 legionella species strains(including 3Lp strains)could be amplified,whereas non legionella stains could not produce positive amplified fragments.The amplified product was validated by semi nested PCR which yielded the 430bp specific fragment of 16SrRNA gene.The 649bp specific fragment of 3 Lp strains could be ampified by mip primers and non Lp stains was negative.Semi nested PCR was used to validate the amplified product,by which the 399bp specific fragment of 3 Lp stains mip gene could be amplified.The sensitivity was 10CFU/ml.The method was used successfully to detect a isolated suspected legionella strain in water sample.Conclusion Semi nested PCR was economy,rapid,simple,sensitive,specific and a powerful tool in early detection,environmental monitoring and control infection outbreaks of legionella." @default.
- W2348835848 created "2016-06-24" @default.
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- W2348835848 date "2002-01-01" @default.
- W2348835848 modified "2023-09-25" @default.
- W2348835848 title "Establishment and Application of Semi-nested PCR for Detection of Legionella Species" @default.
- W2348835848 hasPublicationYear "2002" @default.
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