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- W2348869701 abstract "Objective:To clone and identify the promoter in the mouse SMO gene(mSMO).Method:The total RNA was extracted from NIH 3T3 cells by Trizol reagent.RNA ligase-mediated 5'-RACE was performed to obtain transcriptional start point of mouse SMO gene.Nest PCR was used to clone DNA fragment that contained the promoter of mSMO gene using genome DNA derived from NIH 3T3 cells as the template.Starting from this DNA fragment,11 reporter plasmids that contained 5' serial truncated promoter were constructed.After the plasmids were transiently transfected into Cos7 cells,the luciferase activity were essayed to determine promoter activity.Result:6 transcriptional start points(+1,+4,+27,+31,+51,and +63bp) were identified in the mSMO gene and all of them located in exon 1.A ~2.1 kb DNA fragment upstream the mSMO gene was obtained.Starting from this fragment,11 reporter plasmids with serial truncated promoter in the 5' end were constructed.The reporter gene assay proved the promoter activity for this upstream DNA fragment.When truncated to the positions at-615 and-176bp,two peak values of promoter activity were observed and lowest promoter activity was obtained when the fragment was truncated to the position of-373bp.Conclusion:mSMO gene contains multiple transcriptional start points.The core promoter locates within the area-176 to +124bp and the area between-615 and-176 is the important transcriptional regulation region." @default.
- W2348869701 created "2016-06-24" @default.
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- W2348869701 date "2011-01-01" @default.
- W2348869701 modified "2023-09-23" @default.
- W2348869701 title "Structional and Functional Studies for the Promoter of Mouse Spermine Oxidase Gene" @default.
- W2348869701 hasPublicationYear "2011" @default.
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