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- W2348895199 abstract "Objective To construct expression vectors of human CCR7 gene and to obtain H157 cells that can express CCR7 stably.Methods CCR7 coding domain was amplified from lung adenocarcinoma patient using RT-PCR,directionally cloned into pEGFP-N1 plasmid.The recombinant vectors were transfected into H157 cell lines by DOTAP liposome and screened by G418 selective medium.The expression of CCR7 was verified by RT-PCR and flow cytometry.Results Correct construction of pEGFP-CCR7 was identified by methods of restriction enzyme analysis,PCR amplication and nucleotide-sequencing.CCR7 was found to be expressed in the transfected H157 cells with fluorescent microscopy,RT-PCR and flow cytometry.Conclusions The CCR7-expressing plasmid has been constructed successfully and CCR7 is expressed stably in human adenocarcinoma H157 cells." @default.
- W2348895199 created "2016-06-24" @default.
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- W2348895199 date "2011-01-01" @default.
- W2348895199 modified "2023-09-24" @default.
- W2348895199 title "Construction of human CCR7 gene vector and its stable expression in lung adenocarcinoma H157 cells" @default.
- W2348895199 hasPublicationYear "2011" @default.
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