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- W2348900890 abstract "[Objective]The aim was to explore the effect of using fractional precipitation to remove the non-cellulose from snailase.[Method] Adding ammonium sulphate to crude enzyme,the saturation of ammonium sulphate in the solution was increased gradually from 10% to 100% with the gradient of 10%.The protein content was detected by the coomassie brilliant blue method and the cellulase activity was detected by DNS method.[Result]The impure protein in most non-cellulose from snailase in the original enzyme liquid began to deposit when the saturation of ammonium sulphate reached to 40%,then the specific activity of the obtained deposition was lowest.The cellulase activity in the separated deposition was highest when the saturation was 50%.More cellulase protein were separated when the saturation was 50% and with the several following saturation,which showed that using ammonium sulphate fractional precipitation to deposite snailase could improve the cellulase activity.[Conclusion]Using the saturation of 50% to deposite cellulose from snailase was most rational." @default.
- W2348900890 created "2016-06-24" @default.
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- W2348900890 date "2009-01-01" @default.
- W2348900890 modified "2023-09-25" @default.
- W2348900890 title "Grade Separation of Cellulase from Snailase" @default.
- W2348900890 hasPublicationYear "2009" @default.
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