Matches in SemOpenAlex for { <https://semopenalex.org/work/W2348908465> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2348908465 abstract "The experiment was conducted to insert T7 RNA polymerase(RNAP) gene into cells of swine by retroviral vector system,then to analyze its hereditary stability.The bacteriophage T7 RNAP gene was amplified via PCR from lysogen DE3,and the gene was cloned into pBABEpuro retrovial vector to construct a recombinant plasmid that named as pBABEpuro/T7.Then the pT7BABEpuro and pVSV-G plasmids were cotransfected into GP2-293 packaging cells by Lipfection 2000,some pseudotype viruses were ingathered and transfected into PK15 and SK6 cells under polybrene,respectively.The T7-PK15 and T7-SK6 cells were propagated in DMEM with puromycin respectively.The genome extraction from the cells transfected different times,and the T7 RNAP gene was amplified from the genome by PCR.Expression and activity of T7 RNAP gene in T7-PK15 and T7-SK6 cells were analyzed by immediate fluorescence and SDS-PAGE assay.Results showed that the T7 RNAP gene had been integrated into the chromosome of T7-PK15 and T7-SK6 cells,and expressed stably at high level.After 40 times passage,transcriptional activity of T7-PK15 and T7-SK6 cell lines were not weaked.T7 RNAP gene had been stably integrated into the chromosome of T7-PK15 and T7-SK6 cells,construction of cell lines provided favorable tools for virus rescue in vivo." @default.
- W2348908465 created "2016-06-24" @default.
- W2348908465 creator A5081183372 @default.
- W2348908465 date "2011-01-01" @default.
- W2348908465 modified "2023-09-26" @default.
- W2348908465 title "Stable Expression of T7 RNA Polymerase Gene Mediated by Retroviral Vector in PK15 and SK6 Cells" @default.
- W2348908465 hasPublicationYear "2011" @default.
- W2348908465 type Work @default.
- W2348908465 sameAs 2348908465 @default.
- W2348908465 citedByCount "0" @default.
- W2348908465 crossrefType "journal-article" @default.
- W2348908465 hasAuthorship W2348908465A5081183372 @default.
- W2348908465 hasConcept C104317684 @default.
- W2348908465 hasConcept C127413603 @default.
- W2348908465 hasConcept C153911025 @default.
- W2348908465 hasConcept C22744801 @default.
- W2348908465 hasConcept C2776441376 @default.
- W2348908465 hasConcept C2780048736 @default.
- W2348908465 hasConcept C2780098074 @default.
- W2348908465 hasConcept C54009773 @default.
- W2348908465 hasConcept C54355233 @default.
- W2348908465 hasConcept C547475151 @default.
- W2348908465 hasConcept C78519656 @default.
- W2348908465 hasConcept C86803240 @default.
- W2348908465 hasConceptScore W2348908465C104317684 @default.
- W2348908465 hasConceptScore W2348908465C127413603 @default.
- W2348908465 hasConceptScore W2348908465C153911025 @default.
- W2348908465 hasConceptScore W2348908465C22744801 @default.
- W2348908465 hasConceptScore W2348908465C2776441376 @default.
- W2348908465 hasConceptScore W2348908465C2780048736 @default.
- W2348908465 hasConceptScore W2348908465C2780098074 @default.
- W2348908465 hasConceptScore W2348908465C54009773 @default.
- W2348908465 hasConceptScore W2348908465C54355233 @default.
- W2348908465 hasConceptScore W2348908465C547475151 @default.
- W2348908465 hasConceptScore W2348908465C78519656 @default.
- W2348908465 hasConceptScore W2348908465C86803240 @default.
- W2348908465 hasLocation W23489084651 @default.
- W2348908465 hasOpenAccess W2348908465 @default.
- W2348908465 hasPrimaryLocation W23489084651 @default.
- W2348908465 hasRelatedWork W1822680052 @default.
- W2348908465 hasRelatedWork W2069996913 @default.
- W2348908465 hasRelatedWork W2089166803 @default.
- W2348908465 hasRelatedWork W2349875825 @default.
- W2348908465 hasRelatedWork W2356155935 @default.
- W2348908465 hasRelatedWork W2357504759 @default.
- W2348908465 hasRelatedWork W2358715044 @default.
- W2348908465 hasRelatedWork W2361097685 @default.
- W2348908465 hasRelatedWork W2363255534 @default.
- W2348908465 hasRelatedWork W2364188481 @default.
- W2348908465 hasRelatedWork W2365165092 @default.
- W2348908465 hasRelatedWork W2367301048 @default.
- W2348908465 hasRelatedWork W2369453118 @default.
- W2348908465 hasRelatedWork W2373096866 @default.
- W2348908465 hasRelatedWork W2375219435 @default.
- W2348908465 hasRelatedWork W2376342659 @default.
- W2348908465 hasRelatedWork W2380525608 @default.
- W2348908465 hasRelatedWork W2387559891 @default.
- W2348908465 hasRelatedWork W2388258828 @default.
- W2348908465 hasRelatedWork W3017181318 @default.
- W2348908465 isParatext "false" @default.
- W2348908465 isRetracted "false" @default.
- W2348908465 magId "2348908465" @default.
- W2348908465 workType "article" @default.