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- W2348918829 abstract "[Objective]The paper aimed to study on Electrochemical DNA Biosensor applied for the food safety inspection,and electrochemical DNA biosensor was established.[Method]Introducing epoxy silanization reagent at the electrode of tin-toped indium oxide(ITO),reacting of amination oligonucleotide,nucleic acid probe was covalently immobilized,electrochemical method and x-ray photoelectron spectroscopy(XPS) were characterized.[Result]The electrochemistry cyclic voltammetry of solution with 5 mmol/L K_3Fe(CN)_3 and 50μmol/L Ru(bpy)_3~(2+) scan showed that the epoxy group reagents can be modified into ITO electrode successfully.10μl 0.5μmol/L oligonucleotide was spread at the 0.5 cm~2 electrode,amino functionalized oligonucleotide,the total amount was about 4 pmol,the immobilization rate was 8%。X-ray electron spectroscopy complemented the electrochemical detection,the intensity of the peak of Si2p and N1s had been increased by 38.89%、16.67%.[Conclusion] The covalent DNA immobilization method was stable and robust,and easy to hybridize with its complementary target.The DNA biosensor can be widely used in environmental monitoring and food safety analysis." @default.
- W2348918829 created "2016-06-24" @default.
- W2348918829 creator A5057097136 @default.
- W2348918829 date "2009-01-01" @default.
- W2348918829 modified "2023-09-23" @default.
- W2348918829 title "Preliminary Study on Electrochemical DNA Biosensor Applied for Food Safety Inspection" @default.
- W2348918829 hasPublicationYear "2009" @default.
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