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- W2348934650 abstract "Objective Surgical stress induces a series of endocrine and metabolic changes including glucose metabolism and insulin-resistance. The purpose of the present study was to investigate the changes in glucose transporter-4 (Glut-4) mRNA expression in skeletal muscle after cholecystectomy under epidural block. Methods One gram of the rectus abdominis muscle was taken while abdomen was being opened and closed in patients undergoing cholecystectomy under epidural block. Total RNA of the muscle cells was extracted by trizol one-step template. RT-PCR was used to determine the Glut-4 mRNA amplification products with β-actin mRNA as an internal control. The Glut-4 mRNA expression was expressed by (desired gene/ β-actin×100% . The plasma glucose and insulin levels were determined at the same time. Results Glut-4 mRNA expression was significantly reduced (P0.05) and plasma glucose level increased ( P 0.05 ), while abdomen was being closed as compared with those while abdomen was being opened. Plasma insulin level did not change significantly after operation ( P 0.05 ) . Conclusions The results indicated that the synthesis of Glut-4 is suppressed by surgical stress of cholecystectomy under epidural block leading to insulin resistance." @default.
- W2348934650 created "2016-06-24" @default.
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- W2348934650 date "2002-01-01" @default.
- W2348934650 modified "2023-09-25" @default.
- W2348934650 title "The effect of cholecystectomy under epidural block on glucose transport-4 mRNA expression in skeletal muscle" @default.
- W2348934650 hasPublicationYear "2002" @default.
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