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- W2348936974 abstract "Objective:To investigate the patterns and differences of the rDNA ITS sequences of six traditional Mongolian medicinal plants in Clematis L.(C.aethusifolia,C.macropetala,C.intricata,C.hexapetala C.mandshurica and C.brevicaudata)and to establish a molecular biological method in identification of these plants.Methods: DNA was extracted from the dry leaves of the plants,ITS gene fragments were amplified by PCR and sequenced.The sequences were compared and analyzed using Clustal X and MEGA softwares.Results:The length of ITS region varied from 548~552 bp with 57 variable sites,23 parsimony information sites and 33 singleton sites in the 6 plants in Clematis L.The sequences were submitted to NCBI database with the registry numbers of JN809679-JN809684.Conclusion:ITS sequences could be used to identify the six species in Clematis L.and also could provide a reference for molecular identification." @default.
- W2348936974 created "2016-06-24" @default.
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- W2348936974 date "2012-01-01" @default.
- W2348936974 modified "2023-09-24" @default.
- W2348936974 title "rDNA-ITS SEQUENCE ANALYSIS OF THE TRADITIONAL MONGOLIAN MEDICINAL PLANTS IN CLEMATIS L." @default.
- W2348936974 hasPublicationYear "2012" @default.
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