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- W2348971686 abstract "The coat protein(CP) gene of Cucumber green mottle mosaic virus Liaoning isolate(CGMMV-LN) was amplified by RT-PCR,and ligated to the expression vectors pGEX-4T-3 and pET-22b(+).The recombinant plasmids pGEX-4T-3-CGMMV CP and pET-22b(+)-CGMMV CP were transformed into E.coli BL21 respectively and then induced by IPTG.It was shown that the cp gene was highly expressed by SDS-PAGE and Western blot analysis.The molecular weights of recombinant proteins were 43.8 kDa and 17.3 kDa in pGEX-4T-3 and pET-22b(+) vectors.Antiserum with high specificity was produced after the rabbit was immunized with 17.3 kDa purified recombinant protein,and the titer was 1/20 000 detected by antigen coating plate ELISA(ACP-ELISA)." @default.
- W2348971686 created "2016-06-24" @default.
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- W2348971686 date "2007-01-01" @default.
- W2348971686 modified "2023-09-28" @default.
- W2348971686 title "Expression of cp gene and antiserum preparation of Cucumber green mottle mosaic virus" @default.
- W2348971686 hasPublicationYear "2007" @default.
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