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- W2348974995 abstract "Objective:To establish animal model of experimentally autoimmune myocarditis in BALB/c mice. Methods:Cardiac myosin was isolated from pig heart through methods of differential centrifugation and (NH 4) 2SO 4 fractionation, and was purified by DEAE Sephadex A 50 chromatography, judged by SDS PAGE assay and Western blotting.Then it was used for immunization of genetically predisposed mice to establish animal model of autoimmune myocarditis. Results:On the 14th day after the first immunization with cardiac myosin 97%(35/36), the BALB/c mice developed histological myocarditis with pathological features of infiltration of mononucleocytes,derangement of muscle fibers and even sarcoplasmic lysis.A high titer of myosin specific autoantibodies(IgG) and elevated troponin I level were detected in immunized mice. Conclusion:Cardiac myosin isolated from pig is capable of inducing autoimmune myocarditis in BALB/c mice." @default.
- W2348974995 created "2016-06-24" @default.
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- W2348974995 date "2002-01-01" @default.
- W2348974995 modified "2023-09-25" @default.
- W2348974995 title "Autoimmune myocarditis induced by immunization of BALB/c mice with cardiac myosin" @default.
- W2348974995 hasPublicationYear "2002" @default.
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