Matches in SemOpenAlex for { <https://semopenalex.org/work/W2348975073> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2348975073 abstract "AIM:To develop a method of primary culture of neonatal rat cardiomyocytes with high survival cell rate and high purity.METHODS:Hearts of 1-to 2-day-old neonatal Sprague Dawley rats were removed under sterile conditions with 0.25% trypsin and 0.2% collagenase type Ⅱ at 37℃ under the conditions of graded digestion myocardial tissue.Differential adherence purification was applied.The time was extended to 90 min and the medium was changed after 48 h.RESULTS:Adherent growth of myocardial cells was observed at 2-4 h and pseudopodia growth at 2-3 days.Myocardial cells formed cell clusters and the synchronous beat frequency was about 80-130 times/min with survival rate of( 93.9 ± 2.8) % and purity of( 91.9 ± 2.2) %.CONCLUSION:We developed a method to culture myocardial cells with high survival cell rate and high purity." @default.
- W2348975073 created "2016-06-24" @default.
- W2348975073 creator A5005779417 @default.
- W2348975073 date "2013-01-01" @default.
- W2348975073 modified "2023-09-26" @default.
- W2348975073 title "Culture and identification of cardiomyocytes in neonatal rats" @default.
- W2348975073 hasPublicationYear "2013" @default.
- W2348975073 type Work @default.
- W2348975073 sameAs 2348975073 @default.
- W2348975073 citedByCount "0" @default.
- W2348975073 crossrefType "journal-article" @default.
- W2348975073 hasAuthorship W2348975073A5005779417 @default.
- W2348975073 hasConcept C126322002 @default.
- W2348975073 hasConcept C134018914 @default.
- W2348975073 hasConcept C1491633281 @default.
- W2348975073 hasConcept C153911025 @default.
- W2348975073 hasConcept C16685009 @default.
- W2348975073 hasConcept C181199279 @default.
- W2348975073 hasConcept C185592680 @default.
- W2348975073 hasConcept C2776283816 @default.
- W2348975073 hasConcept C2778394429 @default.
- W2348975073 hasConcept C2780340462 @default.
- W2348975073 hasConcept C2781357212 @default.
- W2348975073 hasConcept C2994367422 @default.
- W2348975073 hasConcept C43617362 @default.
- W2348975073 hasConcept C54355233 @default.
- W2348975073 hasConcept C55493867 @default.
- W2348975073 hasConcept C71924100 @default.
- W2348975073 hasConcept C81885089 @default.
- W2348975073 hasConcept C86803240 @default.
- W2348975073 hasConceptScore W2348975073C126322002 @default.
- W2348975073 hasConceptScore W2348975073C134018914 @default.
- W2348975073 hasConceptScore W2348975073C1491633281 @default.
- W2348975073 hasConceptScore W2348975073C153911025 @default.
- W2348975073 hasConceptScore W2348975073C16685009 @default.
- W2348975073 hasConceptScore W2348975073C181199279 @default.
- W2348975073 hasConceptScore W2348975073C185592680 @default.
- W2348975073 hasConceptScore W2348975073C2776283816 @default.
- W2348975073 hasConceptScore W2348975073C2778394429 @default.
- W2348975073 hasConceptScore W2348975073C2780340462 @default.
- W2348975073 hasConceptScore W2348975073C2781357212 @default.
- W2348975073 hasConceptScore W2348975073C2994367422 @default.
- W2348975073 hasConceptScore W2348975073C43617362 @default.
- W2348975073 hasConceptScore W2348975073C54355233 @default.
- W2348975073 hasConceptScore W2348975073C55493867 @default.
- W2348975073 hasConceptScore W2348975073C71924100 @default.
- W2348975073 hasConceptScore W2348975073C81885089 @default.
- W2348975073 hasConceptScore W2348975073C86803240 @default.
- W2348975073 hasLocation W23489750731 @default.
- W2348975073 hasOpenAccess W2348975073 @default.
- W2348975073 hasPrimaryLocation W23489750731 @default.
- W2348975073 hasRelatedWork W140238201 @default.
- W2348975073 hasRelatedWork W157089593 @default.
- W2348975073 hasRelatedWork W163849424 @default.
- W2348975073 hasRelatedWork W1984994102 @default.
- W2348975073 hasRelatedWork W2052050196 @default.
- W2348975073 hasRelatedWork W2071190310 @default.
- W2348975073 hasRelatedWork W2366312071 @default.
- W2348975073 hasRelatedWork W2409891303 @default.
- W2348975073 hasRelatedWork W2415018387 @default.
- W2348975073 hasRelatedWork W2418244570 @default.
- W2348975073 hasRelatedWork W2419226967 @default.
- W2348975073 hasRelatedWork W2460411039 @default.
- W2348975073 hasRelatedWork W2567164298 @default.
- W2348975073 hasRelatedWork W2949565885 @default.
- W2348975073 hasRelatedWork W2982334237 @default.
- W2348975073 hasRelatedWork W3003107535 @default.
- W2348975073 hasRelatedWork W3030590069 @default.
- W2348975073 hasRelatedWork W3031216929 @default.
- W2348975073 hasRelatedWork W3145359710 @default.
- W2348975073 hasRelatedWork W2814773255 @default.
- W2348975073 isParatext "false" @default.
- W2348975073 isRetracted "false" @default.
- W2348975073 magId "2348975073" @default.
- W2348975073 workType "article" @default.