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- W2348993363 abstract "The FMDV non_structural protein 3B primers were designed and synthesized.The 5' end and 3' end of primers were added the sequence of restriction endonucleases BamH I and HindⅢ respectively.The 3B gene coding region was obtained from the viral genome RNA by RT_PCR.The amplified fragment was cloned into baculovirus transfer vector pFASTIBAC HTa.The recombinant vector was transformated into DH10BAC and cultured in LB yolutes vowtuiing GM,Ka,X_gal and ITPG.The white colonies were selected and Bacmid_3B recombinant vectors were used for transfecting Sf9 cells mediated with CELLFECTIN reagent.After three to four times amplification of the viral stocks,the NPS_3B was expressed in High 5 cells.The SDS_PAGE result showed that recombinant eukaryotic vector was constructed and the NSP_3B was expressed in insect cells successfully.The specafic electrophoretic band in SDS_PAGE(16KD) was noted and authenticated by Western Blotting." @default.
- W2348993363 created "2016-06-24" @default.
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- W2348993363 date "2003-01-01" @default.
- W2348993363 modified "2023-09-25" @default.
- W2348993363 title "Construction and expression of FMDV non-structural protein 3B gene eukaryotic vector" @default.
- W2348993363 hasPublicationYear "2003" @default.
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