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- W2349005871 abstract "The parthenogenetic response of mouse oocytes to ethanol,6-DMAP or both treatment was investigated in this study. Mouse cocytes collected from superovulated mice 18 hours after hGC injection were stimulated with ethanol and 6-DMAP alone or in combination. The results are as follows: 1. Activation percentages of mouse oocytes were 41.3,63.7,57.9 and 85.6%,respectively, when cocytes were treated with 5 and 10 % ethanol for 5 and 10 minutes, indication that in this case, with the increase of ethanol concentration and duration, the oocyte activation percentage increased significantly. 2. When cocytes were cultured in CZB medium containing 2mmol/L 6-DMAP for 2, 4and 6 hours, the activation rates were 12,25 and 40 % , respectively. 3. When cocytes were first treated in 5 % ethanol for 5 min and then cultured with 2mmol/L 6-DMAP for 6h, the activation rate(65.5 % )was significantly higher than that obtained by treatment with 5 % ethanol or 6-DMAP alone. 4. When oocytes were cultutred in CZB with 2mmol/L 6-DMAP for 6 h after treated in 10 % ethanol for 5 min, the activation percentage reached 100 % , markedly higher than that achieved by 10 % ethanol alone. 5. When oocytes were treated with ethanol alone, with the increase of ethanol concentration and duration the percentage of 2-cell-type(immediate cleavage) activiated oocytes increased significantly,but culture with 6-DMAP inhibited immediate cleavage while increased formation of two pronculei." @default.
- W2349005871 created "2016-06-24" @default.
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- W2349005871 date "2002-01-01" @default.
- W2349005871 modified "2023-09-23" @default.
- W2349005871 title "PARTHENOGENETIC ACTIVATION OF MOUSE OOCYTES BY ETHANOL AND 6-DMAP" @default.
- W2349005871 hasPublicationYear "2002" @default.
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