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- W2349007333 abstract "Objective The C35 gene is a novel tumor marker gene that is consistently expressed at high levels in breast cancer cells but not in normal mammary gland epithelial cells.Thus,it can serve as a marker gene in the early diagnosis of breast cancer.In order to discover the mechanism of its regulation and signal pathway in breast cancer,three kinds of yeast two-hybrid bait fusion vectors were constructed for C35,and their transcription activation and cytotoxicity were tested.Methods C35 gene sequences were cloned from T47D cells by RT-PCR,and three kinds of C35 gene fragments were inserted into the bait expression vector pGBKT7.The transcription activation and cytotoxicity of recombinants was determined by selective culture plates and β-galactosidase assay.Results All of the three bait vectors were successfully constructed;of these,the full-length expression product of the C35 gene particularly inhibited the activity of yeast cells.The two fragments(C35 1-153 and C35 154-348) had neither transcription activation nor cytotoxicity.Conclusion The full length of the C35 gene is likely to cause yeast cell death by inhibiting the protein expression systems of those cells.The two fragments lacked this cytotoxicity and thus can be used as a yeast two-hybrid system for T47D cDNA library screening." @default.
- W2349007333 created "2016-06-24" @default.
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- W2349007333 date "2011-01-01" @default.
- W2349007333 modified "2023-10-03" @default.
- W2349007333 title "Construction and transcriptional activation of a C35 bait fusion vector" @default.
- W2349007333 hasPublicationYear "2011" @default.
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