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• W2349037567 abstract "Leaf senescence is considered as one of important factors to decrease ornamental values of foliage plants. In the attempt to study and understand the molecular mechanism of leaf senescence , a senescent leaf cDNA library of Coleus blumei was constructed and a small EST library was obtained. According to the sequence of an EST fragment with a cystathionine beta synthase ( CBS) domain , a novel leaf senescence2 associated gene ( SAG) full2length cDNA encoding a CBS2domain2containing protein , denoted Cbcbs , was rapidly cloned using a strategy of RACE combined with cDNA library. The full length of the Cbcbs gene was 859 bp long (accession No. EF076754) and contained a 609 bp open reading frame (ORF) encoding a 202 amino acid protein. One stop codon ( TAA) was found in 5' UTR and one possible polyadenylation signal , AATAAA , and a pentanucleotide motif , ATTTA , were found in 3'UTR. The CbCBS contained a predicted mitochondrial targeting peptide in the N2terminal region , two conserved and intact CBS domains , four casein kinase Ⅱ (CKⅡ) phosphorylation sites , three protein kinase c ( PKC) phosphorylation sites and one tyrosine sulfation ( TS) site. Sequence comparisons and phylogenetic analysis showed that CbCBS was a novel senescence or stress2associated protein. The prediction analysis of secondary structure and three dimensional structure of CbCBS suggested that the chief function of the protein was decided by the CBS domain pair. The expression pattern of Cbcbs in leaves was analyzed by RT2PCR. It was demonstrated that Cbcbs gene was a senescence2associated gene (SAG) and expressed in all leaf stages , young stage ( Y) being the lowest and terminal senescence stage ( S3) being the highest , and was upregulated along with the leaf senescence. Function analysis showed that the mature CbCBS maybe acts as a sensor of cellular energy status and directly or indirectly regulates cellular energy levels to increase ATP content in mitochondria during periods of metabolic" @default.
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• W2349037567 date "2007-01-01" @default.
• W2349037567 modified "2023-09-23" @default.
• W2349037567 title "Molecular Characterization and Functional Prediction of a Novel Leaf SAG Encoding a CBS-domain-containing Protein from Coleus blumei" @default.
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