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- W2349071736 abstract "J6-1 leukemic cell line was established in our laboratory from AMML patient and its proliferation depends on the cell-cell contact. The J6-1 cell membrane lysate, but not conditioned medium, showed activity for J6-1 cell's proliferation. Recently, the J6-1 membrane associated factor, stimulating proliferation of J6-1 cells, was discovered and designed as MAF-J6-1. After fractionation via Sephadex G-100 and sequential two steps FPLC Mono Q, it was purified over 333-fold. MAF-J6-1 was moderately stable for heat, acid and alkali, the molecular weight was 50 kD as estimated by SDS-PAGE. The neutralization test was performed with the McAb of M-CSF. The result showed that the MAF-J6-1 might be a membrane bound M-CSF.It is suggested that J6-1 leukemic cells might exist contact stimulating autocrine mechanism for proliferation, and the membrane bound M-CSF plays an important role in the contact stimulation." @default.
- W2349071736 created "2016-06-24" @default.
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- W2349071736 date "1994-01-01" @default.
- W2349071736 modified "2023-09-24" @default.
- W2349071736 title "ISOLATION AND CHARACTERIZATION OF MEMBRANE ASSOCIATED REGULATING FACTOR FROM HUMAN LEUKEMIC CELL LINE J6-1" @default.
- W2349071736 hasPublicationYear "1994" @default.
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