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- W2349096800 abstract "Objective:To explore the effects of tumor necrosis factor-β(TNF-β)on damage of hematopoietic cells by CD8+HLA-DR+ effector T cells of the patients with severe aplastic anemia(SAA),and to prove the role of TNF-β in immunopathogenesis of SAA.Methods:CD8+HLA-DR+ T cells as effector cells were sorted from bone marrow mononuclear cells(BMMNC) of SAA patients by magnetic activated cell sorting system(MACS).CD3+ cells MACS depleted BMMNC of normal controls as target cells were co-cultured with the effector cells(1∶1) in different concentrations of TNF-β(0,15,25 and 50 ng/ml) for 72 hours.The percentage of Annexin V positive cells were measured by flow cytometry.The quantitive assay of IL-10,IFN-γ in the culture supernatant was also performed using the enzyme linked immunosorbent assay kit.Results:The apoptotic rate(AR) of target cells in control group and experimental groups were(49.85±20.33)%,(51.65 ±20.34)%,(55.94 ±20.19)% and(57.72 ±19.45)%.AR of target cells in 25 ng/ml and 50 ng/ml TNF-β groups were significiantly higher than those in control group and 15 ng/ml TNF-β group(P0.05).There were no significiant difference between those in control group and 15 ng/ml TNF-β group,also between those in 50 ng/ml and 25 ng/ml TNF-β groups(all P0.05).The levels of IL-10 in the culture supernatant in different groups were(217.99±29.47)ng/ml,(216.47±29.00)ng/ml,(212.15±13.19)ng/ml and(218.01±28.61)ng/ml.There were no difference among them(P0.05).The levels of IFN-γ in the culture supernatant of 50 ng/ml TNF-β group[(593.50±48.18)ng/ml] was significiantly higher than those of control group [(544.85±69.77)ng/ml],15 ng/ml TNF-β group [(567.38±74.51)ng/ml] and 25 ng/ml TNF-β group [(544.05±79.69)ng/ml](P0.05).Conclusion:TNF-β could enhance the apoptosis of hematopoietic cells induced by effector T cells of SAA patients in dose-respanse manner.High level of TNF-β might also promote CD8+HLA-DR+T cell secrete IFN-γ which could collaborative by damage hematopoiesis." @default.
- W2349096800 created "2016-06-24" @default.
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- W2349096800 date "2012-01-01" @default.
- W2349096800 modified "2023-09-25" @default.
- W2349096800 title "Effects of TNF-β on damage of hematopoiesis by CD8~+HLA-DR~+T cells of SAA patients" @default.
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