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- W2349100023 abstract "Objective:To establish CHO cell strain stably expressing human soluble a proliferation-inducing ligand(sAPRIL) protein.Methods:The sAPRIL gene was cloned into lentivirus vector pWPXL to obtain the recombinant plasmid named sAPRIL-pWPXL.Then the vector sAPRIL-pWPXL,envelope plasmid pMD2.G and packaging plasmid psPAX2 were cotransfected into HEK-293T cells to generate the recombinant lentivirus.The viral supernatant on HEK-293T cells was collected and used to infect CHO cells to get the sAPRIL protein expressed stably.The expression product was detected and verified by RT-PCR,Western blot and FACS.Results:sAPRIL gene was successfully cloned and inserted into the lentivirus vector pWPXL.And sAPRIL protein was stably expressed by CHO cells.Conclusion:The sAPRIL protein has been expressed successfully in CHO cells for the further study of its biology function." @default.
- W2349100023 created "2016-06-24" @default.
- W2349100023 creator A5023984232 @default.
- W2349100023 date "2011-01-01" @default.
- W2349100023 modified "2023-09-22" @default.
- W2349100023 title "Establishment and identification of CHO cell strain stably expressing human soluble a proliferation-inducing ligand(sAPRIL) protein" @default.
- W2349100023 hasPublicationYear "2011" @default.
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