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- W2349133091 abstract "A recombinant canine adenovirus that incorporated a fusion protein of EGFP into adenovirus capsid pIX was constructed.Based on the recombinant canine adenovirus plasmid pPolyⅡ-CAV-2,enhanced green fluorescent protein(EGFP) incorporated at the minor capsid protein IX(pIX) without heterologous promoter was constructed.The pEGFP-SIX vector that the 340bp fragment containing IX protein of canine adenovirus type 2 with MluⅠand EcoRⅠwas inserted the pEGFP-C1-dAseⅠplasmid was constructed The EGFP gene was inserted rightward between the last codon of the Ⅸ gene and the stop codon.Then we gained the recombinant plasmids that displayed EGFP gene pPolyII-CAV-2-ⅨE(34.3kb).The results showed that the target genes were cloned into the Ⅸ protein by restriction enzyme digestion." @default.
- W2349133091 created "2016-06-24" @default.
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- W2349133091 date "2008-01-01" @default.
- W2349133091 modified "2023-09-28" @default.
- W2349133091 title "Virus Display Based on Protein IX of Canine Adenovirus Type 2" @default.
- W2349133091 hasPublicationYear "2008" @default.
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