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- W2349202332 abstract "On the base of the sequence of β-glucosidase gene(bgl1) of Aspergillus niger,a specific primer was synthesized.Using the total RNA of A.niger as template,a target gene fragment was amplified with RT-PCR.The gene was cloned into Escherichia coli JM109 using pMD18T Vector.It was proved that the β-glucosidase gene was cloned successfully by identifying the recombinant vector with PCR.The results of sequence showed that the cloned gene fragment was 2583 bp.Compared with the sequence with the β-glucosidase gene in Genbank,the homology of nucleic acid sequence and amino acid sequence could reach 98.88%,99.77 %,respectively.Furthermore,bgl1 gene without signal peptides composed of about 2500 bp was amplified by PCR,which was beneficial for expression of bgl1 in Pichia pastoris in the subsequent work as the target gene could use the signal peptides sequence of recombinant vector to express efficiently." @default.
- W2349202332 created "2016-06-24" @default.
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- W2349202332 date "2007-01-01" @default.
- W2349202332 modified "2023-09-28" @default.
- W2349202332 title "The Gene Clone and Sequence Analysis of β-Glucosidase from Aspergillus Niger" @default.
- W2349202332 hasPublicationYear "2007" @default.
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