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- W2349203240 abstract "Objective:In order to stimulate the growth and the expression of Tumor Necrosis Factor-α(TNF-α) in Escherichia coli,fermentation conditions of transgenic E.coli were optimized.Method:Single factor experiment and orthogonal experiment were conducted to study fermentation conditions such as composition of culture,concentrations of IPTG and fermentation temperature etc.which affect the growth of E.coli and expression of TNF-α.Result:By range analysis and variance analysis,the optimum fermentation conditions were obtained as followed:the glucose 3%,the tryptone 2%,the yeast 4%,the NH4Cl 1%,the concentration of TPTG 0.5mmol/L,revulsive time 4h,initial pH 7.0,fermentation temperature 37℃.Conclusion:By optimizating fermentation conditions,the yield of E.coli and the expression efficiency of TNF-α could be raised 1.34 fold and 4.11 fold respectively,the yield of TNF-α increased from 0.113% to 0.479%,that had been raised 324% as compared with the original yeild." @default.
- W2349203240 created "2016-06-24" @default.
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- W2349203240 date "2008-01-01" @default.
- W2349203240 modified "2023-09-23" @default.
- W2349203240 title "Optimization for Cultivation Conditions of Transgenic Escherichia coli Harbering TNF-α Gene" @default.
- W2349203240 hasPublicationYear "2008" @default.
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